In the MAC induction experiments, mitochondria were isolated from normal (nonapoptotic) cells and then treated with t-Bid. Recall that Bak is constitutively expressed on mitochondria, whereas most Bax is normally found in the cytosol and only moves to mitochondria after truncation of Bid.
There may be higher endogenous levels of Bak than Bax in mitochondria from control (nonapoptotic) cells. These differences might be explained, in part, by Bax having a lower affinity for t-Bid than Bak. (58) who report that recombinant t-Bid was much more effective in triggering cytochrome c release in Bax KO mitochondria by comparison with Bak KO mitochondria. These results are consistent with those of Wei et al. The concentration of t-Bid needed to induce equivalent levels of MAC activity is much greater in Bak-deficient than in Bax-deficient MEF cells, the assembly of MAC channels is slower in the former cells, and the amount of cytochrome c released by t-Bid treatment is less (Fig. Despite the fundamental similarities in the channel behavior of MAC-Bax and MAC-Bak, these channels differ in several important respects. These basic characteristics are independent of Bax and Bak content for MAC. All MACs function by providing cytochrome c with a transport pathway across the outer membrane. MAC has an immense pore that is slightly cation-selective and not voltage-dependent. You can configure CPU affinity in your environment to run Mule in a nonvirtualized multicore server and comply with the number of cores included in your subscription. Many operating systems provide a mechanism to bind a process to one or more central processing units (CPUs) so that the process executes only on the assigned CPUs.
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